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1x thermopol reaction buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs 1x thermopol reaction buffer
    1x Thermopol Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 2335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+reaction+buffer/ThermoPol+Reaction+Buffer/pmc12993374-147-70-74
    Average 98 stars, based on 2335 article reviews
    1x thermopol reaction buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Purification:

    Article Title: A cis -acting ligase ribozyme generates circular RNA in vitro for ectopic protein functioning
    Article Snippet: : 2 U/μg RNA, Cat#ab286929, abcam) or 2 mg/ml RNase A (Cat#12091021, Invitrogen) for a 1 h incubation at 37 °C. .. For the 3’ end tailing of polyA, equal amounts of purified RNA were buffered in 1X reaction buffer with 1 mM ATP and 5 U of E. coli PolyA Polymerase (Cat#M0276S, NEB) in a 20 μl reaction. ..

    Incubation:

    Article Title: Molecular Characterization of Tobacco Necrosis Virus A Variants Identified in Sugarbeet Roots
    Article Snippet: .. Briefly, 3.0 μg of linearized DNA was incubated with 1x reaction buffer, 100 U T7 RNA polymerase (New England Biolabs), 10 mM dithiothreitol (Thermo Fisher Scientific, Waltham, MA), 1 mM ribonucleotide mix (New England Biolabs) and 80 U RNAse inhibitor murine (New England Biolabs) according to manufacturer’s instructions. ..

    other:

    Article Title: Structural Insights into an Antiparallel Chair‐Type G‐Quadruplex From the Intron of NOP56 Oncogene
    Article Snippet: Primer extension was performed for 2 h at 37 °C in 1X reaction buffer (100 m m KCl, 50 m m NaCl, 10 m m Tris‐HCl pH 7.9, 10 m m MgCl 2 and 1 m m DTT) containing 50 μ m primer‐template, 1.5 m m dNTPs (New England Biolabs), and 0.3 U/μL KF with 5′ to 3′ polymerase and 3′ to 5′ exonuclease activities (New England Biolabs).

    Synthesized:

    Article Title: Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency
    Article Snippet: The Cas12f-MG119-28 nuclease mRNA sequence was codon optimized for human expression (Twist), then synthesized and cloned into a high copy ampicillin plasmid (Twist Biosciences). .. Synthesized constructs encoding T7 promoter, UTRs, nuclease ORF, and NLS sequences were digested from the Twist backbone with HindII and BamHI (NEB) and ligated into a pUC19 plasmid backbone with T4 DNA ligase and 1X reaction buffer (NEB). ..

    Construct:

    Article Title: Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency
    Article Snippet: The Cas12f-MG119-28 nuclease mRNA sequence was codon optimized for human expression (Twist), then synthesized and cloned into a high copy ampicillin plasmid (Twist Biosciences). .. Synthesized constructs encoding T7 promoter, UTRs, nuclease ORF, and NLS sequences were digested from the Twist backbone with HindII and BamHI (NEB) and ligated into a pUC19 plasmid backbone with T4 DNA ligase and 1X reaction buffer (NEB). ..

    Plasmid Preparation:

    Article Title: Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency
    Article Snippet: The Cas12f-MG119-28 nuclease mRNA sequence was codon optimized for human expression (Twist), then synthesized and cloned into a high copy ampicillin plasmid (Twist Biosciences). .. Synthesized constructs encoding T7 promoter, UTRs, nuclease ORF, and NLS sequences were digested from the Twist backbone with HindII and BamHI (NEB) and ligated into a pUC19 plasmid backbone with T4 DNA ligase and 1X reaction buffer (NEB). ..



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